《生命科学》 2021, 33(3): 292-302
通讯作者：刘如娟 , Email:email@example.com
Post-transcriptional modifications widely exist in various RNA molecules and are essential for RNA function. At present, there are more than 160 kinds of chemical modifications on RNA (https://iimcb.genesilico.pl/modomics/), and methylation is the most common type of modification. Traditional RNA detection methods, such as thin layer chromatography, high performance liquid chromatography and mass spectrometry, have made important contributions to the identification and quantification of RNA modifications. However, the precise identification of RNA modifications and the detection of modifications on low abundant RNA molecules rely on some high-throughput sequencing based techniques, which have been developed in recent years. Traditional RNA detection methods and some high-throughput sequencing based methods have been introduced in the article “The detection methods of RNA modifications” published in this journal in 2018. Here, we summarize the popular detection methods of RNA modification based on high-throughput sequencing, and introduce their working principles and application.
Communication Author：LIU Ru-Juan , Email:firstname.lastname@example.org