中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2008, 20(2): 190-
tRNA核酸内切酶的研究进展
杨 景,于莹莹,黄 鹰*
(南京师范大学生命科学学院, 南京210046)
摘 要:tRNA在蛋白质合成过程中起着极其重要的作用。在所有的生物体内,tRNA首先以前体形式转录,然后必需经过一系列的加工后才能成为有功能的tRNA分子。tRNase Z、RNase P和tRNA剪接内切酶是参与tRNA前体加工的三种主要的核酸内切酶,分别参与tRNA前体3{$39}末端、 tRNA前体5{$39}末端和内含子剪接的加工。这三种酶具有不同的结构特征,并且利用完全不同的催化机制水解磷酸二酯键。tRNase Z和RNase P都是金属酶,活性中心分别需要Zn2+和Mg2+的参与;而tRNA剪接内切酶活性中心不需要金属离子,是一个由不同催化亚基上的关键氨基酸残基构成的组合式活性中心。
关键词:tRNA;tRNase Z;RNase P; tRNA剪接内切酶
中图分类号:Q55;Q783.1  文献标识码:A
 
Progress on study of tRNA endonucleases involved in pre-tRNA processing
YANG Jing, YU Ying-ying, HUANG Ying*
(School of Life Sciences, Nanjing Normal University, Nanjing 210046, China)
Abstract: tRNA plays a crucial role in protein synthesis. In all organisms, tRNAs are synthesized as precursors. To be functional, pre-tRNAs must undergo a number of processing steps. tRNase Z,RNase P and tRNA splicing endonuclease are three major endonucleases that participate in pre-tRNA processing. These three endonucleases have distinct structural features and use completely different catalytic mechanisms to hydrolyze the phosphodiester bond. tRNase Z and RNase P are metalloenzymes and their active centers require Zn2+and Mg2+,respectively. The active center of tRNA splicing endonuclease does not require metal ions, and is a composite active center consisting of key residues from different catalytic subunits.
Key words: tRNA; tRNase Z; RNase P; tRNA splicing endonuclease
 
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