中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2007, 19(5): 557-
RNAi在药物研究中的应用
王丽娜,袁崇刚*
(华东师范大学生命科学学院,上海200062)
摘 要:RNA干扰是双链RNA分子在mRNA水平上诱发的序列特异性的转录后基因表达沉默。在哺乳动物细胞里,RNAi可以由21-25个核苷酸长度的小干扰RNA (siRNA)触发,在后基因组时代的基因功能研究和药物开发中具有广阔的应用前景。现针对近年RNAi在药物研究中的应用包括应用RNAi发现新药靶、辅助确认药靶、RNAi药物、RNAi与耐药性等方面作一综述。
关键词:RNAi;药物靶点;高通量筛选;RNAi药物;多药耐药性
中图分类号:R522; R965  文献标识码:A
 
Development and applications of RNAi technology in drug research
Wang Lina, Yuan Chonggang*
(College of Life Science, East China Normal University, Shanghai 200062, China)
Abstract: RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) induces sequence-specific posttranscriptional gene silencing of homologous transcripts. In mammalian cells, RNAi can be triggered by 21-25 nucleotide duplexes of small interfering RNA (siRNA). In the postgenome era, RNAi has broad application prospect in probing gene function and drug research. This review summarises development and applications of RNAi technology in drug research,such as drug-target discovery and identification, RNAi reagent, multidrug resistance over the past few years.
Key words: RNA interference; drug target; high throughput screening; RNAi reagent; multidrug resistance
 
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