中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2013, 25(7): 735-741
基因组定点编辑技术的研究进展
张智辉,董少忠*,寸 韡*
(中国医学科学院&北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明 650118)
摘 要:基因组编辑是建立在基因靶向修饰的基础上,对生物基因组进行改造的一项新技术。通过利用人工核酸酶ZFn、TALEN 和细菌获得性免疫系统CRISPR,可在靶位点制造 DNA 双链切口进而诱导细胞内源性修复机制,通过同源重组修复或非同源末端连接途径实现基因敲除、替换和纠正。对目前3 个主要的基因组定点编辑技术的应用和发展作一综述。
    关键词:定点基因组编辑;锌指核酸酶;TALEN ;CRISPR/Cas9
 
Progress in targeted genome editing technologies
ZHANG Zhi-Hui, DONG Shao-Zhong*, CUN Wei*
(Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Kunming 650118, China)
Abstract: Genome editing is a novel technology based on precise genome-targeting modification to enable systematic reverse engineering of causal genetic variation. A set of gene editing techniques, zinc finger nucleases,transcription activator-like effector nucleases and bacterial CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immunity system, have been used to cleave the double-strand DNA at specific site, followed by endogenous DNA repair. These techniques, therefore, make precise changes to the DNA of living cell by, for example, knocking out a gene, correcting a genetic mutation or replacing DNA fragment at the specific site. The new progress in targeted genome editing technologies was discussed in this paper.
    Key words: targeted genome editing; zinc finger nucleases; TALEN; CRISPR/Cas9
 
首页 | 刊物简介 | 编委会 | 投稿须知 | 广告业务 | 过刊浏览 | 联系我们
中国科学院上海生命科学信息中心《生命科学》编辑部
Copyright © 2012-2015 《生命科学》编辑部 All Rights Reserved.
沪ICP备05033115号-30
您是第2978091 位访问者,欢迎!