中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2007, 19(1): 97-
癌症差异蛋白质组学研究中样品分离和鉴定分析技术
李选文,陈 平,张丽军,梁宋平*
湖南师范大学生命科学学院 蛋白质化学与蛋白质组学研究室,长沙410081
摘 要:随着人类基因组测序的完成,癌症研究的重点从基因组学转移到蛋白质组学研究中。癌症研究中的差异蛋白质组学技术也飞速发展,包括癌症样品制备、分离,蛋白质鉴定分析、蛋白质组定量研究和翻译后修饰研究等。这些技术极大地推动了与癌症相关的差异蛋白质组学研究,使蛋白质组学在癌症早期诊断、治疗,监测以及发现新药物治疗靶标方面发挥更大的作用。本文主要综述了近年来癌症差异蛋白质组学研究中样品分离和鉴定分析技术。
关键词:癌症;差异蛋白质组;二维电泳;色谱;定量蛋白质组
 
Separation and identification progress in cancer comparative proteomics
LI Xuanwen, CHEN Ping, ZHANG Lijun, LIANG Songping*
The Laboratory of Protein Chemistry and Proteomics, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Abstract: With the human genome sequence now determined, the field of cancer research is moving beyond genomics to proteomics. The comparative strategies involving in the cancer proteomic research have been developed, including cancer sample preparation, proteins or peptides separation, protein identification, bioinformatics, protein quantification and so on. All these will accelerate the identification of cancer markers that could be important in early detection, diagnosis and monitoring efficacy of treatment, and also the development of protein-based, cancer-specific targets, which have a great effect on cancer medicine. In this review, we focused on the sample separation and identification progress in cancer comparative proteomics.
Key words: cancer; comparative proteomics; two-dimensional electrophoresis; liquid chromatography; quantification proteomics
 
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