中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2010, 22(10): 985-990
自组装蛋白质芯片——功能蛋白质组学的新工具
艾润娜,赵晓航*
(中国医学科学院/北京协和医学院肿瘤研究所,分子肿瘤学国家重点实验室,北京100021)
摘 要:传统的蛋白质芯片制备需要进行繁琐的蛋白质表达与纯化。同时,由于蛋白质活性不稳定,蛋白质芯片不宜长期保存。新一代自组装蛋白质芯片,利用无细胞表达体系和DNA固定技术,能够将蛋白质即时、原位表达并固定在芯片上,有效地解决了传统蛋白质芯片的制备和保存问题。目前自组装蛋白质芯片已初步用于大规模蛋白-蛋白质相互作用的筛选,以及鉴定免疫优势抗原等研究。该文介绍了近年自组装蛋白质芯片技术的进展和应用研究。
关键词:自组装蛋白质芯片;蛋白-蛋白质相互作用;无细胞蛋白质合成
中图分类号:Q816  文献标识码:A
 
Self-assembling protein microarray—a new tool for functional proteomics
AI Run-na, ZHAO Xiao-hang*
(State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China)
Abstract: Traditional protein microarrays require time-consuming processes of protein production and purification since the protein activities are not stable and the array products could not be stored properly for a long time. Self-assembling protein microarrays utilized cell-free expression system and DNA immobilization technique,  which allowed multiple-protein expression simultaneously and in situ immobilization on the surface of arrays. This technology effectively overcomes the problems of traditional protein microarrays, and provides a useful tool for functional proteomics analysis. Currently, self-assembling protein microarrays are primarily used for screening protein-protein interactions, identifying immunodominant antigens and so on. This review focused on the recent advancement and application research on self-assembling protein microarrays.
Key words: self-assembling protein microarray; protein-protein interaction; cell-free protein synthesis
 
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