中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2010, 22(9): 837-845
供体细胞与哺乳动物体细胞核移植
王维, 李运生, 曹鸿国, 张运海
(安徽农业大学动物科技学院,安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036)
摘 要:哺乳动物体细胞核移植(克隆)技术在转基因动物生产、珍稀动物资源复原与保护、生物学基础研究等方面业已显示出重要的应用价值,而目前该技术还与诱导多能干细胞技术一同被认为是创制患者特异性多能干细胞,为再生医学临床“细胞治疗”提供素材的最佳手段。但是,体细胞克隆的效率仍不理想,关键机制还不清楚,严重制约了该技术的推广。因此,如何提高克隆效率已成为人们普遍关心的首要问题。在体细胞克隆技术所涉及的各环节中,供体细胞是影响克隆效率的最关键因素之一。该文从供体细胞的生物学因素和技术因素两方面进行了回顾,旨在为进一步探寻建立物种或供体细胞个性化准备方案,为提高动物克隆效率提供参考。
关键词:体细胞核移植;重编程;供体细胞
 
Lessons learned from effect of donor cells?on efficiency of somatic cell nuclear transfer in mammals
WANG Wei, LI Yun-sheng, CAO Hong-guo, ZHANG Yun-hai*
(Anhui Provincial Laboratory of Local Domesitic Animal Genetic Resource Conservation and Biological Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China)
Abstract: Somatic cell nuclear transfer (cloning) has shown its important practical values in aspects of animal transgenesis, recovery and pretection of rare or endangered animal species, and biological basic researches, and especially this technique, together with induced pluripotent stem cell technology, is recognized as a most ideal tool to provide potential tailored patient specific pluripotent stem cells for cell therapies in human regenerative medicine clinics. However, unfortunately, poor cloning efficiency and unclear understanding of key mechanisms underlying nuclear reprogramming seriously limit wider uses of animal cloning. Thus, improvement of the cloning efficiency seems to be cloner抯 priority. Donor cells are regarded as one of the most essential factors among those influencing outcome of mammalian cloning. Herein, this paper reviewed lessens learned from somatic donors?effect on animal cloning effciency, specifically from the biological and technological aspects of donor cells, to help better our understanding of setting up species- or donor cell type- specific protocols for increasing cloning efficacy.  
Key words: somatic cell nuclear transfer; reprogramming; donor cells
 
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