| LC-MS在植物代谢组学分析中的应用 |
| 常玉玮*，王国栋* |
| (中国科学院遗传与发育生物学研究所，北京 100101) |
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| 摘 要：利用植物代谢组学研究植物体系受刺激或扰动后的代谢变化，可以揭示植物面对外界环境变化或基因变化时的应答机制。液相色谱- 质谱联用(LC-MS) 技术由于其高分辨能力、高灵敏度，适合高沸点、热不稳定及高分子量化合物的检测等优点而在植物代谢组学研究中发挥重要作用。近些年来，LC-MS 在诸多方面取得了较快发展，如快速LC-MS、纳流LC-MS、二维LC-MS、衍生化LC-MS 等。随着分析方法的发展和完善，基于LC-MS 的植物代谢组学已成功应用于代谢表型差异、转基因植物安全性评价、生物及非生物胁迫、植物基因功能鉴定、辅助育种等多个研究方向。 |
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| Application of LC-MS in plant metabolomics |
| CHANG Yu-Wei*， WANG Guo-Dong* |
| (Institute of Genetics and Developmental Biology， Chinese Academy of Sciences， Beijing 100101， China) |
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| Abstract: Plant metabolomics helps to illustrate the mechanisms underlying plant responses to environment change or gene mutations at the level of metabolites. Liquid chromatography-mass spectrometry (LC-MS) is characteristic of high resolution power and high sensitivity. Furthermore， LC-MS is suitable for the analysis of metabolites with high boiling point or high molecular weight or metabolites that are not thermostable. Based on these advantages，LC-MS analysis plays an important role in plant metabolomics. LC-MS has been significantly improved in several aspects in recent years， including fast LC-MS， nano LC-MS， 2D LC-MS， derivatization LC-MS etc. With the development of LC-MS technology， plant metabolomics has been successfully applied in many fields， including phenotypic discrimination， safety assessment of transgenic plants， biotic or abiotic stress study， identification of plant gene function， crop breeding etc. |
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