中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
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《生命科学》 2015, 27(5): 631-639
黑腹果蝇二元表达系统原理及应用
陈轮号1,刘怿君2*
(1 浙江大学医学院临床医学系,杭州 310058;2 浙江大学医学院神经生物学系,杭州 310058)
摘 要:二元表达系统是简单、高效的遗传操作工具,提高了特定基因的重组效率,实现了基因精确时空表达。目前应用于黑腹果蝇的二元表达系统主要有GAL4/UAS、FLP/FRT、LexA/lexAop、Q 系统以及Cre/loxP 、CRISPR/Cas9 系统,其中GAL4/UAS 和FLP/FRT 系统应用最为广泛。二元表达系统的联合成功应用于嵌合体的构建,为细胞谱系分析、细胞间相互作用等研究提供了强有力的工具。综述了果蝇二元表达系统的原理、应用及各系统之间的联合应用,为研究者选择遗传操作工具提供了参考。
 
The principle and applications of binary expression systems in Drosophila melanogaster
CHEN Lun-Hao1, LIU Yi-Jun2*
(1 Department of Clinical, School of Medicine, Zhejiang University, Hangzhou 310058, China; 2 Department of Neurobiology, School of Medicine, Zhejiang University, Hangzhou 310058, China)
Abstract: Binary expression systems are simple and ideal genetic techniques, which have increased efficiency of gene recombination and allowed the precise manipulation of gene expression in a temporal and spatial fashion. Binary expression systems widely used in Drosophila melanogaster at present include GAL4/UAS, FLP/FRT, LexA/lexAop, Q system, Cre/loxP, and CRISPR/Cas9 system, and among them, GAL4/UAS system and FLP/FRT system are most widely used. The combination of these systems has succeeded in establishing mosaic, providing
    powerful techniques for studies in cell lineage analysis, interaction among cells and so on. This review summarizes the principle and applications of binary expression systems in Drosophila melanogaster and further introduces the
    co-applications among these systems. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for researchers.
 
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