中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
“健康与疾病的免疫”国际学术研讨会通知      关于有网站冒充本刊网站的声明
《生命科学》 2014, 26(4): 392-399
介导siRNA传递的非病毒载体及其研究进展
李泽豪1,任小元1,王世兵1,孔祥东1,2*
(1 浙江理工大学生命科学学院生物材料研究所,杭州 310018;2 浙江理工大学材料与
    纺织学院,教育部先进纺织材料和制备技术重点实验室,杭州 310018)
RNA 干扰(RNAi) 是一种疾病治疗的新途径,其中小干扰RNA (siRNA) 可破坏mRNA 的完整性,抑制疾病相关基因的表达,而开发安全有效的siRNA 传递载体仍面临巨大挑战。相较于病毒载体系统,非病毒载体系统安全性高、负载量大、生物相容性好,更适用于疾病治疗。综述了可用于siRNA 传递的一些非病毒载体( 包括脂质体、细胞穿膜肽、水凝胶、树枝状大分子、磷酸钙、碳纳米管等),阐述了载体与siRNA 的结合,靶向疾病组织或细胞并诱导特异基因沉默的机制、瓶颈问题等。
 
Research advances in non-viral-based siRNA delivery systems
LI Ze-Hao1, REN Xiao-Yuan1, WANG Shi-Bing1, KONG Xiang-Dong1,2*
(1 Institute of Biomaterials, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2 Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education,College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China)
RNA interference (RNAi) is a new mechanism triggered by small interfering RNA (siRNA), which represents a promising gene therapy strategy by destroying the integrity of the complementary mRNA in the cytoplasm, causing sequence-specific posttranscriptional gene silencing. Systemic siRNA therapy is hampered by inefficient delivery of its carrier. Compared with viral-based delivery systems, non-viral-based delivery systems are safer, and have higher load, greater cellular compatibility, so they are more suitable for clinical application. In general, the non-viral carriers include cation lipid-based carriers, cation cell-penetrating peptides, dendrimers, cation polymers, inorganic nanomaterials. This review describes the recent types and advantage in siRNA delivery using non-viral-based siRNA delivery systems.
 
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