中国科技核心期刊
CN:31-1600/Q
ISSN:1004-0374
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《生命科学》 2014, 26(3): 202-206
非编码RNA研究技术概述
苟兰涛1,刘默芳1,付向东2*
(1中国科学院上海生命科学研究院生物化学与细胞生物学研究所,分子生物学国家重点实验室,上海 200031;2 Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA)
非编码RNA 是当前生命科学研究领域的前沿热点。系统地研究生物体内非编码RNA 的起源、结构、功能和作用机制,有助于我们更加全面、深入地了解基因表达调控机理及生物体的生命活动,而科学问题的解答依赖于实验技术和实验方法的创新和革命。本文按不同的实验目的和研究内容,就当前非编码RNA领域内主要的研究策略和方法技术作一简述。
 
Unique approaches to unique problems in ncRNA research
GOU Lan-Tao1, LIU Mo-Fang1, FU Xiang-Dong2*
(1 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; 2 Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA)
Non-coding RNAs have been the hot frontier of life sciences in recent years. Systematic studies of the origins, structures, biological function and their mechanisms of non-coding RNAs will provide new avenues to help us understand the complicated mechanisms of gene regulation as well as cellular activities. Since scientific breakthrough highly relies on technology innovations, here we summarize the main strategies and techniques in non-coding RNA research.
 
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